2022:

  1. Reith P, Braam S, Welkenhuysen N, Lecinski S, Shepherd J, MacDonald C, Leake MC, Hohmann S, Shashkova S, Cvijovic M. The Effect of Lithium on the Budding Yeast Saccharomyces cerevisiae upon Stress Adaptation. Microorganisms. (2022) In Press https://doi.org/10.3390/microorganisms10030590

  2. Schnitzer B, Welkenhuysen N, Leake MC, Shashkova S, Cvijovic M. The effect of stress on biophysical characteristics of misfolded protein aggregates in living Saccharomyces cerevisiae cells. Exp Gerontol (2022) In Press https://doi.org/10.1016/j.exger.2022.111755

2021:

  1. .Jin X, Lee J-E, Schaefer C, Luo X, Wollman AJM, Tian T, Zhang X, Chen X, Li Y, McLeish TCB, Leake MC, Bai F. Membraneless organelles formed by liquid-liquid phase separation increase bacterial fitness. Science Adv (2021) 7:eabh2929.

  2. Backer AS, King GA, Biebricher AS, Shepherd JW, Noy A, Leake MC, Heller I, Wuite GJL, Peterman EJG. Elucidating the Role of Topological Constraint on the Structure of Overstretched DNA Using Fluorescence Polarization Microscopy. J Phys Chem B (2021) 125(30):8351-8361.

  3. Leake MC. Correlative approaches in single-molecule biophysics: a review of the progress in methods and applications. Methods. (2021). 193:1-4.

  4. Shepherd JW, Higgins EJ, Wollman AJM, Leake MC. PySTACHIO: Python Single-molecule TrAcking stoiCHiometry Intensity and simulatiOn, a flexible, extensible, beginner-friendly and optimized program for analysis of single-molecule microscopy data. Comput Struct Biotechnol J. (2021) 19:4049-4058.

  5. Yoshua SB, Watson GD, Howard JAL, Velasco-Berrelleza V, Leake MC, Noy A. Integration host factor bends and bridges DNA in a multiplicity of binding modes with varying specificity. NAR (2021) 49:8684-98.

  6. Shepherd JW, Payne-Dwyer AL, Lee J-E, Syeda A, Leake MC. Combining single-molecule super-resolved localization microscopy with fluorescence polarization imaging to study cellular processes. J. Phys. Photonics. (2021) I 3:034010.

  7. Saldivia M, Wollman AJM, Carnielli JBT, Jones NG, Leake MC, Bower-Lepts C, Rao SPS, Mottram JC. A CLK1-KKT2 signaling pathway regulating kinetochore assembly in Trypanosoma brucei. MBio. (2021) 15:e0068721.

  8. Dienerowitz M, Howard JAL, Quinn SD, Dienerowitz F, Leake MC. Single-molecule FRET dynamics of molecular motors in an ABEL trap. Methods. (2021) S1046-2023(21)00024-4 doi: 10.1016/j.ymeth.2021.01.012

  9. Laidlaw KME, Bisinski DD Shashkova S, Paine KM, Veillon MA, Leake MC, MacDonald C. A glucose starvation response governs endocytic trafficking and eisosomal retention of surface cargoes. Journal of Cell Science. (2021) 134(2):jcs257733.

2020:

  1. Shepherd JS, Lecinski S, Wragg J, Shashkova S, MacDonald C, Leake MC. Molecular crowding in single eukaryotic cells: using cell environment biosensing and single-molecule optical microscopy to probe dependence on extracellular ionic strength, local glucose conditions, and sensor copy number. Methods. (2020) S1046-2023(20)30236-X. doi: 10.1016/j.ymeth.2020.10.015.

  2. Shashkova S, Nyström T, Leake, MC, Wollman AJM. Correlative single-molecule fluorescence barcoding of gene regulation in Saccharomyces cerevisiae. Methods. (2020) S1046 2023(20)30224-3. doi: 10.1016/j.ymeth.2020.10.009.

  3. Velasco-Berrelleza V, Burman M, Shepherd JW, Leake MC, Golestanian R, Noy A. SerraNA: a program to determine nucleic acids elasticity from simulation data. Phys Chem Chem Phys. (2020) 22(34):19254-19266.

  4. Juan-Colás J, Dresser L, Morris K, Lagadou H, Ward RH, Burns A, Tear S, Johnson S, Leake MC, Quinn SD. The Mechanism of Vesicle Solubilization by the Detergent Sodium Dodecyl Sulfate. Langmuir. (2020) 6(39):11499-11507.

  5. Dresser L, Hunter P, Yendybayeva F, Hargreaves AL, Howard JAL, Evans GJO, Leake MC, Quinn SD. Amyloid-β oligomerization monitored by single-molecule stepwise photobleaching. Methods. (2020) S1046-2023(20)30084-0. doi: 10.1016/j.ymeth.2020.06.007.

  6. Cosgrove J, Novkovic M, Albrecht S, Pikor NB, Zhou Z, Onder L, Mörbe U, CupovicJ, Miller H, Alden K, Thuery A, O’Toole P, Pinter R, Jarrett S, Taylor E, Venetz D, Heller M, Uguccion M, Legler DF, Lacey CJ, Coatesworth A, Polak WG, Cupedo T, Manoury B, Thelen M, Stein JV, Wolf M, Leake MC, Timmis J, Ludewig B, Coles MC. 2020. B cell Zone Reticular Cell Microenvironments Shape CXCL13 Gradient Formation. Nature Communications. (2020) 11:3677.

  7. Miller H, Contera S, Wollman A, Hirst A, Dunn KE, Schroeter S, O’Connell D, Leake M. 2020. Biophysical characterization of DNA origami nanostructures reveals inaccessibility to intercalation binding sites. Nanotechnology. (2020) 31(23):235605.

  8. Shepherd J, Greenall R, Probert MI, Noy A, Leake MC. 2020. Emergence of Sequence-dependent structural motifs in stretched, torsionally constrained DNA. Nucleic Acids Research (2020) 48:1748-1763.

  9. Wollman AJM, Hedlund E, Shashkova S, Leake MC. 2020. Towards mapping the 3D genome through high speed single-molecule tracking of functional transcription factors in single living cells. Methods 170:82-89.

2019:

  1. Dalgarno PA, Juan-Colás J, Hedley GJ, Piñeiro L, Novo M, Perez-Gonzalez C, Samuel IDW, Leake MC, Johnson S, Al-Soufi W, Penedo JC, Quinn SD. Unveiling the multi-step solubilization mechanism of sub-micron size vesicles by detergents. Sci Rep. 2019 9(1):12897.

  2. Sun, Y, Wollman, AJM, Huang, F, Leake MC, Luning, L. Single-organelle quantification reveals the stoichiometric and structural variability of carboxysomes dependent on the environment. The Plant Cell (2019) 31(7):1648-1664.

  3. Syeda, A, Wollman, AJM, Hargreaves, AL Howard J, Brüning, J-L, McGlynn, P, Leake MC*.Single-molecule live cell imaging of Rep reveals the dynamic interplay between an accessory replicative helicase and the replisome. Nucleic Acids Research. (2019) (12):6287-6298.

  4. Pu, Y, Li, Y, Jin, X, Tian, T, Ma, Q, Zhao, Z, Lin, S-L, Chen, Z, Li, B, Yao, G, Leake MC, Lo, C-L, Bai F. ATP-dependent dynamic protein aggregation regulates bacterial dormancy depth critical for antibiotic tolerance. Mol Cell. (2019) 7:143-156.

  5. Stracy, M, Wollman, AJM, Kaja, E, Gapinski, J, Lee, J-L, Leek, VA, McKie, SJ, Mitchenall, LA, Maxwell, A, Sherratt, DJ, Leake MC, Zawadzki, P. Single-molecule imaging of DNA gyrase activity in living Escherichia coli. Nucleic Acids Research. (2019) 47:210-220.

  6. Haapasalo, K, Wollman, AJM, de Haas, C, van Kessel, K, van Strijp, J, Leake MC. Staphylococcus aureus toxin LukSF dissociates from its membrane receptor target to enable renewed ligand sequestration. FASEB J. (2019) 33:3807-3824.

2018:

  1. Hoogenboom B, Leake MC. The case for biophysics super-groups in physics departments. Physical Biology. (2018) 15(6):060201.

  2. Miller H, Cosgrove J, Wollman AJM, Emily Taylor, Zhaokun Z, O’Toole P, Coles M, Leake MC. High-speed single-molecule tracking of CXCL13 in the B-follicle. Frontiers in Immunology. (2018) 9:1073.

  3. Leake MC. Transcription factors in eukaryotic cells can functionally regulate gene expression by acting in oligomeric assemblies formed from an intrinsically disordered protein phase transition enabled by molecular crowding. Transcription. (2018) 9:1-9.

  4. Lund VA, Wacnik K, Turner RD, Cotterell BE, Walther CG,, Fenn SJ, Grein F, Wollman AJM, Leake MC, Olivier N, Cadby A, Mesnage S, Jones S, Foster S, Molecular coordination of Staphylococcus aureus cell division. Elife. (2018) 7:e32057.

  5. Shashkova S, Wollman AJM, Hohmann S, Leake MC. Characterising maturation of GFP and mCherry of genomically integrated fusions in Saccharomyces cerevisiase. Bio-protocol. (2018) In Press DOI: 10.21769/BioProtoc.2710.

  6. Miller H, Zhou Z, Shepherd J, Wollman A, Leake M. Single-molecule techniques in biophysics: a review of the progress in methods and applications. Rep Prog Phys. (2018) 81(2):024601.

2017:

  1. Shashkova S, Leake MC. Systems biophysics: single-molecule optical proteomics in single living cells. Curr Opin Syst Biol (2017) In Press.

  2. Wollman AJM, Shashkova S, Hedlund EG, Friemann R, Hohmann S, Leake MC. Transcription factor clusters regulate genes in eukaryotic cells ELife (2017) 6:e27451.

  3. Shashkova S, Leake MC. Single-molecule fluorescence microscopy review: shedding new light on old problems. Biosci Rep. (2017)37(4). pii: BSR20170031. doi: 10.1042/BSR20170031.

  4. Carrascosa JL, Leake MC. Imaging the Cell. Biophys Rev. (2017) In Press doi: 10.1007/s12551-017-0280-8.

  5. Shashkova S, Wollman AJM, Leake MC, Hohmann S. 2017 The yeast Mig1 transcriptional repressor is dephosphorylated by glucose-dependent and -independent mechanisms. FEMS Microbiol Lett. (2017) Aug 1;364(14). doi: 10.1093/femsle/fnx133.

  6. Beattie,T. Hapadia, N, Nicolas E, Uphoff S, Wollman AJM, Leake, MC, Reyes‑Lamothe, R. Frequent exchange of the DNA polymerase during bacterial chromosome replication. eLife (2017) 6:e21763.

  7. Dunn, KE, Leake, MC, Wollman, AJM, Trefzer, MA, Johnson, S, Tyrrell, AM. An experimental study of the putative mechanism of a synthetic autonomous rotary DNA nanomotor. R Soc Open Sci (2017) 4:160767.

2016:

  1. Wollman, AJM, Miller, H, Foster, S, Leake, MC. An automated image analysis framework for segmentation and division plane detection of single live Staphylococcus aureus cells which can operate at millisecond sampling time scales using bespoke Slimfield microscopy. Phys Biol. (2016) 13(5):055002.

  2. Zhou, Z & Leake, MC. Force spectroscopy in studying infection. Adv Exp Med Biol. (2016) 915:307-27.

  3. Miller, H, Wollman, AJM, Leake, MC. Designing a single-molecule biophysics tool for characterizing DNA damage for techniques that kill infectious pathogens through DNA damage effects. Adv Exp Med Biol. (2016) 915:115-27.

  4. Wollman, AJM, Syeda, AH, McGlynn, P, Leake, MC. Single-molecule observation of DNA replication repair pathways in E. coli. Adv Exp Med Biol. (2016) 915:5-16.

  5. Leake, MC. The biophysics of infection. Adv Exp Med Biol. (2016) 915:1-3.

  6. Badrinarayanan, A & Leake, MC. Using fluorescence recovery after photobleaching (FRAP) to study dynamics of the structural maintenance of chromosome (SMC) complex in vivo. Methods Mol Biol. (2016) 1431:37-46.

  7. Wollman, AJM & Leake, MC. Single molecule narrowfield microscopy of protein-DNA binding dynamics in glucose signal transduction of live yeast cells. Methods Mol Biol. (2016) 1431:5-15.

  8. Leake, MC. New advances in Chromosome Architecture. Methods Mol Biol. (2016) 1431:1-3.

  9. Lenn, T, Leake, MC. Single-molecule studies of the dynamics and interactions of bacterial OXPHOS complexes. Biochimica et Biophysica Acta (2016) 1857(3):224-31.

2015:

  1. Cordes T, Moerner W, Orrit M, Sekatskii S, Faez S, Borri P, Prabal Goswami H, Clark A, El-Khoury P, Mayr S, Mika J, Lyu G, Cross D, Balzarotti F, Langbein W, Sandoghdar V, Michaelis J, Chowdhury A, Meixner AJ, van Hulst N, Lounis B, Stefani F, Cichos F, Dahan M, Novotny L, Leake M, Yang Frsc H. Plasmonics, Tracking and Manipulating, and Living Cells: general discussion. Faraday Discussions (2015) 184:451-73.

  2. Wollman, AJM, Leake, MC. Millisecond single-molecule localization microscopy combined with convolution analysis and automated image segmentation to determine protein concentrations in complexly structured, functional cells, one cell at a time. Faraday Discussions (2015) 184:401-24.

  3. Zhou , Z, Miller, H, Wollman, AJM, Leake, MC. Developing a new biophysical tool to combine magneto-optical tweezers with super-resolution fluorescence microscopy. Photonics (2015) 2(3), 758-772.

  4. Wollman, AJM, Nudd R, Hedlund EG, Leake, MC. From Animaculum to single-molecules: 300 years of the light microscope. Open Biol. (2015) 5(4),150019.

  5. Miller, H, Zhou, Z, Wollman, AJM, Leake, MC. Superresolution imaging of single DNA molecules using stochastic photoblinking of minor groove and intercalating dye. Methods (2015) 88, 81-88.

  6. Wollman, AJM, Miller, H, Zhou, Z, Leake, MC. Probing DNA interactions with proteins using a single-molecule toolbox: inside the cell, in a test tube, and in a computer. Biochem. Soc. Trans. (2015) 43(2), 139-145.

2014:

  1. Bryan, SJ, Burroughs, NJ, Shevela, D, Yu, J, Rupprecht, E, Liu, L-N, Mastroianni, G, Xue, Q, Llorente-Garcia, I, Leake, MC, Eichacker, LA, Schneider, D, Nixon, PJ, Mullineaux, CW. Dynamic behaviour and interactions of the Vipp1 protein 1 in cyanobacteria. Mol. Microbiol. (2014) 94(5), 1179–1195.

  2. Nenninger, A, Mastroianni, G, Robson, A, Lenn, T, Xue, Q, Leake, MC, Mullineaux, CW . Independent mobility of proteins and lipids in the plasma membrane of Escherichia coli. Mol. Microbiol. (2014) 92(5):1142-53.

  3. Leake, MC. Analytical tools for single-molecule fluorescence imaging in cellulo. Phys. Chem. Chem. Phys. (2014) 16(25):12635-47.

  4. Llorente-Garcia, I, Lenn T, Erhardt, H, Harriman, OL, Liu, L-N, Robson, A, Chiu, S-W, Matthews, S, Willis, NJ, Bray, CD, Lee, S-H, Shin, JY, Bustamante, C, Liphardt, J, Friedrich, T, Mullineaux, CW, Leake, MC . Single-molecule in vivo imaging of bacterial respiratory complexes indicates delocalized oxidative phosphorylation. Biochimica et Biophysica Acta (2014) 1837:811-24.

2013:

  1. Chiu S-W, Roberts MAJ, Leake MC, Armitage JP. Positioning of chemosensory proteins and FtsZ through the Rhodobacter sphaeroides cell cycle. Mol Microbiol. (2013) 90, 322-37.

  2. Harriman O, Leake MC. A systems-level approach to single-molecule live-cell fluorescence microscopy. Infocus (2013) 30, 5-18.

  3. Leake MC. The Physics of Life: one molecule at a time. Phil Trans B. (2013) 368, 20120248.

  4. Robson A, Burrage K & Leake MC. Inferring diffusion in single live cells at the single molecule level. Phil Trans B. (2013) 368, 20130029.

2012:

  1. Badrinarayanan A, Reyes-Lamothe R, Uphoff S, Leake MC & Sherratt DJ. In vivo architecture and action of bacterial structural maintenance of chromosome proteins. Science (2012) 338, 528-531.

  2. Lenn T & Leake MC. Experimental approaches for addressing fundamental biological questions in living, functioning cells with single molecule precision. Open Biol. (2012), 2, 120090. [Featured Article of the Month].

2011:

  1. Harriman OLJ & Leake MC. Single molecule experimentation in biological physics: exploring the living component of soft condensed matter one molecule at a time. J. Phys.: Condens. Matter (2011), 23, 503101.

  2. Chiu S-W & Leake MC. Functioning nanomachines seen in real-time in living bacteria using single-molecule and super-resolution fluorescence imaging. Int. J. Mol. Sci. (2011)12, 2518-2542.

2010:

  1. Leake MC. Shining the spotlight on functional molecular complexes: the new science of single-molecule cell biology. Commun Integr Biol. (2010), 3, 415-418.

  2. Reyes-Lamothe R, Sherratt DJ & Leake MC. Stoichiometry and architecture of active DNA replication machinery in Escherichia coli. Science (2010), 328, 498-501.

  3. Delalez NJ, Wadhams GH, Rosser G, Xue Q, Brown MT, Dobbie IM, Berry RM, Leake MC & Armitage JP. Signal-dependent turnover of the bacterial flagellar switch protein FliM. Proc Natl Acad Sci U S A 107, 11347-11351 (2010).

  4. Xue Q, Jones NS & Leake MC. A general approach for segmenting elongated and stubby biological objects: extending a chord length transform with the radon transform. Proc. IEEE Internat. Symp. Biomed. Imaging (ISBI): From Nano to Macro (2010), 161-164. (available at http://ieeexplore.ieee.org/stamp/stamp.jsp?arnumber=05490388 DOI: 10.1109/ISBI.2010.5490388).

2009:

  1. Dobbie IM, Robson A, Delalez N & Leake MC. Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy. (2009) J Vis Exp 31, 1508 (2009). Available at: www.jove.com/index/Details.stp?ID=1508, DOI: 10.3791/1508.

  2. Plank M, Wadhams GH & Leake MC . Millisecond timescale slimfield imaging and automated quantification of single fluorescent protein molecules for use in probing complex biological processes. Integr. Biol. 1, 602 – 612 (2009). Available at http://xlink.rsc.org/?doi=B907837A), DOI: 10.1039/b907837a.

  3. Pilizota T, Brown M, Leake MC, Branch R, Berry RM & Armitage JPA. A molecular brake, not a clutch, stops the Rhodobacter sphaeroides flagellar motor. Proc Natl Acad Sci U S A. 106, 11582-11587 (2009).

  4. Xue Q & Leake MC . A novel multiple particle tracking algorithm for noisy in vivo data by minimal path optimization within the spatio-temporal volume. Proc. IEEE Internat. Symp. Biomed. Imaging (ISBI): From Nano to Macro (2009), 1158-1161, (available at http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=5193263&isnumber=5192959) DOI: 10.1109/ISBI.2009.5193263

2008:

  1. Lenn T, Leake MC & Mullineaux CW. In vivo clustering and dynamics of cytochrome bd complexes in the Escherichia coli plasma membrane. Mol. Microbiol. 70, 1397-1407. (2008).

  2. Leake MC, Greene NP, Godun RM, Granjon T, Buchanan G, Chen S, Berry RM, Palmer T & Berks BC. Variable stoichiometry of the TatA component of the twin-arginine protein transport system observed by in vivo single-molecule imaging. Proc Natl Acad Sci U S A. 105, 15376-15381 (2008).

  3. Lenn T, Leake MC & Mullineaux CW. Are Escherichia coli OXPHOS complexes concentrated in specialised zones within the plasma membrane? Biochem. Soc. Trans., 36, 1032-1036 (2008).

2007:

  1. Lo CJ, Leake MC, Pilizota T & Berry RM. Single-cell measurements of Membrane Potential, Sodium-Motive Force and Flagellar Motor Speed in Escherichia coli. Biophys. J. 93, 294-302 (2007).

2006:

  1. Leake MC , Chandler JH, Wadhams GH, Bai F, Berry RM & Armitage JP. Stoichiometry and turnover in single, functioning membrane protein complexes. Nature. 443, 355-8 (2006).

  2. Reid SW, Leake MC, Chandler JH, Lo CJ, Armitage JP, Berry RM. The maximum number of torque-generating units in the flagellar motor of Escherichia coli is at least 11. Proc Natl Acad Sci U S A. 103, 8066-71 (2006).

  3. Leake MC , Grutzner A, Kruger M & Linke WA. Mechanical properties of cardiac titin’s N2B-region by single-molecule atomic force spectroscopy. J Struct Biol. 155, 263-72 (2006).

  4. Bullard B, Benes V, Tzintzuni G, Leake MC, Linke WA & Oberhauser AF. The molecular elasticity of the insect flight muscle proteins projectin and kettin. Proc. Natl. Acad. Sci. U S A. 103, 4451-6 (2006).

  5. Lo CJ, Leake MC & Berry RM. Fluorescence measurement of intracellular sodium concentration in single Escherichia coli cells. Biophys. J. 90, 357-65 (2006).

2005:

  1. Sowa Y, Rowe AD, Leake MC, Yakushi T, Homma M, Ishijima A & Berry RM. Direct observation of steps in rotation of the bacterial flagellar motor. Nature. 437, 916-919 (2005).

  2. Lo CJ, Leake MC & Berry RM. Intracellular sodium concentration of chimera Escherichia coli. FEBS J. 272, 345-6 (2005).

2004:

  1. Linke WA. & Leake MC. Multiple sources of passive stress relaxation in muscle fibres. Phys. Med. Biol. 49, 3613-3627 (2004).

  2. Makarenko I, Opitz CA, Leake MC, Neagoe C, Kulke M, Gwathmey JK, del Monte F, Hajjar RJ & Linke WA. Passive stiffness changes of compliant titin isoforms in human DCM hearts. Circ. Res. 95, 708-16 (2004).

  3. Leake MC , Wilson D, Gautel M & Simmons RM. The elasticity of single titin molecules using a two-bead optical tweezers assay. Biophys. J. 87, 1112-1135 (2004).

  4. Opitz CA, Leake MC, Makarenko I, Benes V & Linke WA. Developmentally regulated switching of titin size alters myofibrillar stiffness in the perinatal heart. Circ. Res. 94, 967-75 (2004).

  5. Bullard B, Ferguson C, Minajeva A, Leake MC, Gautel M, Labeit D, Ding L, Labeit S, Horwitz J, Leonard KR & Linke WA. Association of the chaperone {alpha}B-crystallin with titin in heart muscle. J. Biol. Chem. 279, 7917-7924 (2004).

2003:

  1. Opitz CA, Kulke M, Leake MC, Neagoe C, Hinssen H, Hajjar RJ & Linke WA. Damped elastic recoil of the titin spring in myofibrils of human myocardium. Proc. Natl. Acad. Sci. U S A. 100, 12688-93 (2003).

  2. Leake MC , Wilson D, Bullard B & Simmons RM. The elasticity of single kettin molecules using a two-bead laser-tweezers assay. FEBS Lett. 535, 55-60 (2003).

  3. Rowe A, Leake MC, Morgan H & Berry RM. Rapid rotation of micron and sub-micron dielectric particles measured using optical tweezers. J. Mod. Opt. 50, 1539-1555 (2003).

2002:

  1. Leake MC & Berry RM. 2002. Torque-speed relationship of the flagellar rotary motor of Rhodobacter using an electrorotation technique. Biophys. J. 82, 1954 (2002).